Osidation von DNA-gebundenen Petiden und Proteinen durch DNA-übertragene Ladungstransferprozesse

• Antragsteller: Professor Dr. Hans-Achim Wagenknecht
• Fachliche Zuordnung: Organische Molekülchemie - Synthese, Charakterisierung
• Förderung: Förderung von 2000 bis 2001
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5252952

DNA charge transport chemistry has been the subject of considerable interest with possible consequences in areas as diverse as mutagenesis and molecular electronics. The goal of this project was to put DNA-mediated charge transfer processes in a biological context. In this project, two different approaches were made to investigate how peptides and proteins influence or modulate charge transfer processes through the DNA. In the first part, small DNA-binding peptides were tethered convalently to the 5`-terminus of oligonucleotides. Charge transfer processes were photoinitiated by an intercalating ruthenium photooxidant using the flash-quench technique. In the second part, charge transport and radical trapping were examined in DNA assemblies in the presence of site-specifically bound DNA-interacting enzymes such as cytosin methyltransferase "Hhal" mutants and alkyladenine glycosylase. Charge migration through the DNA base stack has been probed both spectroscopically to observe the formation of radical intermediates and biochemically to assess irreversible oxidative DNA damage. Protein-dependent DNA charge transport is observed over 50 A with guanine radicals. Given the time scale and distance regime such protein-dependent DNA charge transport chemistry requires consideration physiologically.

• DFG-Verfahren: Emmy Noether-Auslandsstipendien