Project Details
Projekt
The kinetochore protein Slk19 interacts with the proteasome: Function of the Slk19-proteasome complex in control of meiosis and mitosis
Applicant
Privatdozent Dr. Wolfgang Hilt (†)
Subject Area
Biochemistry
Term
from 2000 to 2004
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 5250134
Highly sophisticated protease complexes, the proteasomes, are the major cellular tool for regulatory protein degradation. Proteasomal pathways play essential roles in control of the cell division cycle: Proteasome mediated steps are implicated in control of cyclin dependent kinases (CDKs) as well as of other CDK unrelated regulators of the cell cycle. Such processes are required for instance to control start of a new cell cycle as well as entry and exit from mitosis. We have identified a component of the mitotic spindle, Slk19 that interacts with the proteasome subunit Pre4. We could show that Slk19 is required for correct chromosome segregation during meiosis and mitosis. Aims of the proposed projects are to analyze the function of Slk19 in more detail. We will investigate the role of Slk19 in mitotic and meiotic chromosome segregation and if these functions depend on its interaction with the proteasome. We will search for other components linked to Slk19-proteasome function including potential substrates of Slk19 bound proteasomes. Furthermore, we will test whether Slk19 modulates the proteasome. Taken together the planned experiments will provide answer to the question if Slk19 recruites the proteasome to mediate local regulatory degradation steps and how such mechanisms contribute to control of chromosome segregation.
DFG Programme
Priority Programmes
