Struktur und Funktion von LG-Modulen des Laminins (Structure and function of LG modules of laminins)

• Applicant: Dr. Rupert Timpl (†)
• Subject Area: Dermatology
• Term: from 2000 to 2005
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5244266

The proposed project plans the comprehensive analysis of the structure-function relationships of LG modeules of laminin a1 to a5 chains and of the proteoglycan perlecan. These LG modules will be obtained by recombinant production in mammalian cells and used for the mapping of binding sites important for cellmatrix interactions and the supramolecular organization of basement membranes. The ligands examined will be integrin and a-dystroglycan receptors, bacterial receptors, sulfatides, heparin/heparan sulfate and some matrix proteins. Site-directed mutagenesis and X-ray structural analyses will be used for a precise mapping of binding epitopes on various LG modules.

• DFG Programme: Priority Programmes
• Subproject of: SPP 1086: Genetic and Molecular Analysis of Basement Membranes and Basement Membrane Anchorage