Biological function of selenoprotein P: Binding characteristics and cellular localization

• Applicant: Professor Dr. Helmut Sies
• Subject Area: Biochemistry
• Term: from 2000 to 2007
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5237252

Among the selenoproteins, selenoprotein P (SeP) is particularly rich in selenocysteine (8-10 SeCys). Since this protein also contains poly-histidine domains and a high number of lysine residues, capable of binding to glycosaminoglycans, e g heparan sulfates, a strategically interesting positioning on the cell surface was proposed as a basis for a protective function on endothelial cells. The project is to characterize the interaction of SeP with artificially generated surfaces, endothelial cells and other cell types. Potentiation of a protective function against peroxynitrite and other oxidants by preferential localisation of the protein is to be examined. This question is important for evaluating the role of SeP in inflammatory processes and oxidative stress in general.

• DFG Programme: Priority Programmes
• Subproject of: SPP 1087: Selenoproteine - Biochemische Grundlagen und klinische Bedeutung
• Participating Person: Professor Peter Brenneisen, Ph.D.