5-Laser Multiparameter Spectral Flow-Cytometer

• Subject Area: Microbiology, Virology and Immunology
• Term: Funded in 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 509546995

The multi-parameter spectral analyser is the next generation of flow cytometers, allowing to record the entire emitted fluorescence spectrum. By mathematical deconvolution of the signal into the individual emission spectra of the fluorochromes used, it is possible to examine a considerably higher number of parameters simultaneously than before. Such a spectral analyser, fully equipped with five lasers, is able to measure up to 40 parameters in parallel. In this way, a large amount of information can be obtained from samples that are, for example, only available in limited cell numbers or need to be differentiated into many different cell populations. Thus, in patient material or material from animal experiments, with the help of such a multi-parameter spectral analyser, the most diverse cell populations within a cell mixture can be characterised in detail, with a previously unattainable density of information. This functionality supports ideally the research foci of the UKSH, precision and inflammation medicine, whereby the high-resolution phenotyping of the most diverse cell populations represents an important basis for precision medicine approaches. As part of the newly established Cytometry Core Facility, it will be possible to provide access to this innovative instrument for a wide range of academic users at UKSH, CAU and beyond, and to actively support them in their work. A comparable device is not yet available at the Kiel site, so the acquisition of this device will have a positive impact on the academic landscape in Kiel and will form a distinguishing feature of the Cytometry Core Facility and thus support its establishment.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: 5-Laser Multiparameter Spektralzytometer
• Instrumentation Group: 3500 Zellzähl- und Klassiergeräte (außer Blutanalyse), Koloniezähler
• Applicant Institution: Christian-Albrechts-Universität zu Kiel
• Leader: Professor Dr. Alexander Scheffold