Insights into lipopolysaccharide (LPS) transport by solid-state NMR spectroscopy (P01)

• Subject Area: Structural Biology
• Term: since 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 450648163

Lipopolysaccharides (LPS) are the main component of the asymmetric outer membrane of Gram-negative bacteria and protect them against antibiotics and the mammalian immune system. One essential step in maintaining this asymmetry is the translocation of core-LPS across the inner membrane by the ABC floppase MsbA from where LPS is carried on towards the outer membrane by the lipopolysaccharide transport (LPT) system. In project P01, we will address the LPS transport mechanism and explore how its presence alters membrane properties by solid-state NMR.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1507: Protein Assemblies and Machineries in Cell Membranes
• Major Instrumentation: MAS-NMR probe
• Instrumentation Group: 1790 Spektrometer (Massen-, NMR-, außer 170-178)
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Head: Professor Dr. Clemens Glaubitz