Proteomic analysis of brown adipocyte organelle dynamics and contact site formation (P16)

• Subject Area: Cell Biology ; Biochemistry ; Endocrinology, Diabetology, Metabolism
• Term: since 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 450149205

In BAT, mitochondria and LDs form extensive contact sites to orchestrate lipid metabolism. To date, the LD-mitochondrial contact site proteome, its dynamics and regulation remains elusive. We established an organelle proteomics workflow to generate a global map of protein expression, modification and localization upon induction of thermogenesis. We will combine proteomics with cryo-electron tomography and super-resolution microscopy to study the architecture of these contact sites. Combining state-of-the-art techniques, this project will unravel the organelle proteomes and enable studies on dynamic organelle interaction in BAT.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 333: Brown and Beige Fat – Organ Crosstalk, Signaling and Energetics (BATenergy)
• Applicant Institution: Rheinische Friedrich-Wilhelms-Universität Bonn
• Project Head: Dr. Natalie Krahmer