Flowcytometer for high dimensional analytics (64 channels) and multiwell uptake

• Subject Area: Medicine
• Term: Funded in 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 491149881

Flow cytometric technology has become a "main workhorse" on the Biederstein campus of the Klinikum rechts der Isar. In addition to blood analyzes, cells from tissue that have previously been brought into single-cell suspension are also increasingly being analyzed. The limiting factor in immunological research is and will remain the limited access to human cell material and therefore the number of available colors is extremely important for all researchers at the Biederstein campus. The now possible expansion to up to 64 colors is therefore really a fundamental step forward. An important upcoming challenge is the research of cells that are isolated from the tissue, brought into single cell suspension and characterized with flow cytometry using surface markers. Furthermore functional parameter based on secreted products allow functional conclusions. Tissue cells and innate immune cells of the tissue (brushings of the respiratory tract, sputum, skin biopsies, intestinal biopsies) are diverse (dendritic cells, innate lymphocytes, gamma delta-T cells). Basal stem cell analysis of airway and skin barrier place high demands on molecular differentiation with a small number of cells. In addition, there is strong autofluorescence in epithelial cells that can now be easily managed and even utilised for cell identification.The existing device was also acquired at the time through a DFG large-scale device application - the necessity of this equipment for the campus was beyond question. Unfortunately, the old device (after almost 10 years of use) no longer works reliably, causes immense maintenance costs and the lasers are also at the end of their life. However, the requested device is (in addition to the lower operating costs and replacement of the old one) also an important innovation element, as it removes technical limitations, opens up new research possibilities and will ultimately facilitate research with limited cell material and thus new impulses for clinical research at the Biederstein campus will put.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Durchflusszytometer für hochdimensionale Messungen
• Instrumentation Group: 3500 Zellzähl- und Klassiergeräte (außer Blutanalyse), Koloniezähler
• Applicant Institution: Technische Universität München (TUM)
• Leader: Professor Dr. Carsten B. Schmidt-Weber