Transmembrane Signaling in Bacterial Adenylate Cyclases (B08)

• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2007 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 32152271

The quorum-sensing receptors from Vibrio cholerae and Legionella pneumophila have six transmembrane ¿-helices, known ligands and regulate histidine kinases. The membrane anchors are identical in design as those of many adenylate cyclases. Ligand regulation by quorum-sensors was achieved in respective chimeric cyclases. We use bioinformatics, biochemical and physiological methods (cluster and structure analysis recombinant proteins, adenylate cyclase and ß-galaktosidase assays, MacConkey agar plates, FRET analysis) to study the mechanism of quorum-sensor transmembrane signaling. The data shall serve as a prelude to identify ligands of bacterial orphan receptors.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 766: The Bacterial Cell Envelope: Structure, Function and Infection Interface
• Applicant Institution: Eberhard Karls Universität Tübingen
• Project Heads: Professor Dr. Joachim E. Schultz; Professor Dr. Thilo Stehle, until 6/2015