Protein and phosphorylation nuclear dynamics in time and space and its modulation by metabolism (A32*)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

In this project we will study how the molecular mechanism of the circadian clock in the nucleus is aided by systemic and/or tissue specific co-regulators and how this is impaired by metabolic disruption. We will employ mass spectrometry (MS)-based quantitative proteomics to characterize and compare daily proteome and phosphoproteome oscillations in soluble and chromatin enriched nuclear fractions in three different organs of mice fed with normal or high fat diet (HFD). In addition, by using chromatin immunoprecipitation with MS we will analyze how HFD rewires the molecular composition of core clock complexes at the chromatin to disrupt circadian tissue homeostasis.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professorin Maria S. Robles Martinez, Ph.D.