Interplay between tRNA splicing and tRNA modifications (A06)

• Subject Area: Biochemistry
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

tRNAs are transcribed as precursors that require maturation, including non-conventional splicing and modification for function in translation. Here, modifications will be investigated with respect to tRNA processing, starting with reconstitution of enzymatic steps in vitro. Effects of unmodified/misprocessed tRNA will then be evaluated in vivo, as to how structural constraints establish a hierarchy in modification-processing interplay. Finally, modification patterns and fate of tRNAs upon ablation of introns, and formation of tRNA intronic circular RNAs will be characterized.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Heads: Dr. Jirka Peschek; Dr. Francesca Tuorto