Improving intravital microscopy of inflammatory cell response by active motion compensation using controlled adaptive optics (A06)

• Subject Area: Biophysics
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 431460824

We will advance multiphoton fluorescence microscopy by developing a novel optical module comprised of a high-speed deformable mirror that will actively compensate tissue motion during intravital imaging, for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. To control this module in real-time, we will develop mathematical methods that track and predict tissue deformation. This will allow imaging of inflammatory processes at cellular resolution without mechanical tissue fixation.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1450: Multiscale imaging of organ-specific inflammation
• Applicant Institution: Universität Münster
• Project Heads: Professor Dr. Thomas Huser; Professor Dr. Benedikt Wirth