Nanoseond Ultra-Rapid Freeze Quenching for Time-resolved Structural Biology

• Applicant: Professor Dr. Harald Schwalbe
• Subject Area: Structural Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 451906961

The application aims to develop instrumentation for the time-resolved studies of biochemical processes, yielding 3D molecular movies recorded with atomic resolution in real time. Application targets regular and custom-made equipment for pre-steady-state triggering, sampling, probing and detection, including optical, cryo-sedimentation and ultra-high vacuum systems, UHPLC sample delivery and the computation cluster upgrade. Such instrumentation will be essential for time-resolved structural biology, in particular solid state NMR and cryo-EM.

• DFG Programme: New Instrumentation for Research
• Major Instrumentation: Cryo-Iyophilization