In vivo assessment of liver function, perfusion and fat distribution in the animal model by using MRI

• Applicant: Professor Dr. Jürgen R. Reichenbach
• Subject Area: General and Visceral Surgery
• Term: since 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 436883643

Within the overarching goal of the research unit QuaLiPerF to gain a data-integrated, multi-scale understanding of the interplay between hepatic perfusion and function and its recovery after extended hepatectomy (ePHx), this project is devoted to experimental MRI investigations in rats. It aims to establish a comprehensive MR imaging toolbox for small animal imaging at 9.4T and to provide spatially resolved, multi-parametric quantitative in vivo information on liver size, function, and perfusion under various experimental conditions. The toolbox will be applied to scan rats before and after portal vein ligation (PVL) and partial hepatectomy. Animals are monitored longitudinally during regeneration. In addition, the effects of a fat diet on the different MRI parameters, used to characterize the liver status, will also be investigated longitudinally. The acquired MRI data serve to monitor and analyze the physiological adaptations induced by the diet, after PVL and during regeneration of the remnant liver after ePHx. Together with complementary experimental and clinical data contributed by the other projects within the research unit, they form an important source of information for comparison with the temporal changes predicted by the mathematical models, which are also developed in the corresponding projects of the research unit. The data collected in this project will be passed on to be integrated into models on different scales based on statistical approaches. Upon completion of the first funding period of the research unit, a substantial body of experimental in vivo data will be available, which together with the iterative computational modeling and data integration will allow a better prediction of the recovery of liver function.

• DFG Programme: Research Units
• Subproject of: FOR 5151: Quantifying Liver Perfusion-Function Relationship in Complex Resection - A Systems Medicine Approach (QuaLiPerF)
• Co-Investigator: Dr. Karl-Heinz Herrmann