Project Details
Projekt
High-resolution mass spectrometer and liquid chromatography system
Subject Area
Medicine
Term
Funded in 2020
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 446850913
The main research direction of the Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics of the Leipzig University (Medical Faculty) is vascular dysfunction in the context of metabolic diseases. The research activities focus on: 1) basic research of coagulation protease dependent signaling in vascular and metabolic diseases and 2) clinical mass spectrometry, with a focus on targeted analysis of metabolites and bioactive lipids in blood, in cardiovascular and metabolic diseases. For further development and future close interactions of the current projects we urgently need the ability to conduct discovery-driven (untargeted) analyses with high resolution, high sensitivity and high-throughput analyzers. Technology currently available to the working groups (triple-quadrupole mass spectrometers) does not support comprehensive unbiased experiments. Therefore, we apply for a state-of-the-art mass spectrometer that will facilitate this type of analyses. Overall objectives of our vascular-disease and metabolism related projects, which will profit from the discovery-based instrumentation, can be summarized as follows:- Identification and validation of the cell-specific PAR3 (proteinase-activated receptor 3) interactomes in vascular disease and sterile inflammation- Identification of platelet-trophoblast surface interacting proteins in preeclampsia- Identification and validation of nuclear binding partners of p45 NF-E2 (transcription factor p45 NF-E2) and NLRP3 (NACHT, LRR and PYD domains-containing protein 3) proteins in trophoblast cells in placental vascular diseases- Identification and validation of XBP1 (X-box-binding protein 1) interactome in hyperglycemia-induced endoplasmic reticulum stress and cellular metabolism in podocytes in diabetic nephropathy- Characterization of signaling pathways through which cold-shock proteins regulate vascular dysfunction and renal injury- Characterization of signaling pathways through which the redox regulator p66Shc protein regulates hyperglycemia-dependent accelerated atherosclerosis- Detection and quantification of lipid species from macrophage-derived extracellular vesicles- Identification of potential lipid biomarkers and lipid pathways associated with acute-on-chronic liver failure- Lipidomics profiling of atherosclerotic plaques derived from patients with peripheral artery disease or carotid artery plaques- Detection and quantification (lipidomics and proteomics) of neuronal dysfunction (mouse brain) driven by nutrition and obesityBesides of these current objectives, we are intending to extend already existing collaborations in the future, using the options provided by the innovative instrumentation we are applying for. In conclusion, utilization of discovery-based approach will advance our science and enable top-edged research of existing and future projects.
DFG Programme
Major Research Instrumentation
Major Instrumentation
Hochauflösendes Massenspektrometer und Flüssigchromatographiesystem
Instrumentation Group
1700 Massenspektrometer
Applicant Institution
Universität Leipzig
