2D-UHPLC

• Subject Area: Molecular Chemistry
• Term: Funded in 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 435368536

A powerful chromatographic separation method is indispensable for the analysis of the product mixtures often occurring in synthetically-driven research projects. The HPLC methods available in our department are unfortunately reaching their limits of applicability; particularly, for the analysis of polyarenes and for the separation of the mixtures of products obtained in our late-stage functionalization projects. The use of UHPLC on the one hand increases the separation efficiency, and on the other, the elution over a second stationary phase allows the direct evaluation of the enantiomeric excesses obtained. This is an immense advantage over the currently available methods in our department, because the direct determination of the enantioselectivity of a catalytic process might now be done directly from the crude reaction mixtures. So far, each product must be laboriously purified and subsequently eluted again over a chiral stationary phase, which makes the screening for suitable reaction conditions extremely time consuming and expensive. All polyarenes analyzed in our department show fluorescence, which facilitates the analysis of these samples with the fluorescence detector requested. For the non-fluorescent samples, in particular rather polar biomolecules, as well as the products obtained after application of late-stage functionalization protocols, the switchable ESI-MS detector serves for doubtless analysis of the fractions obtained. The isolation and characterization of reaction intermediates and by-products is often very useful for the elucidation of reaction mechanisms and the optimization of reaction conditions; therefore, the requested UHPLC device will be also equipped with an analytical fraction collector, which is so far not available in our institute. In that way, even the smallest amount of substances detected could be isolated and subsequently characterized by, for example, NMR, single-crystal diffractometry, or mass spectrometry.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: 2D-UHPLC
• Instrumentation Group: 1350 Flüssigkeits-Chromatographen (außer Aminosäureanalysatoren 317), Ionenaustauscher
• Applicant Institution: Georg-August-Universität Göttingen
• Leader: Professor Dr. Manuel Alcarazo