Nanoscale organization of adaptor scaffolds involved in polarized cargo sorting at the trans-Golgi network (TGN) (A25*)

• Subject Area: Cell Biology
• Term: from 2019 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 184695641

In project A25 we will investigate the sub-compartmental nanodomain organization of the trans-Golgi network (TGN) that likely underlies the spatio-temporally controlled sorting of distinct cargos to different organelles or plasma membrane domains. Harnessing CRISPR/Cas9 knock-in cell lines for live-cell super-resolution imaging and acute protein degradation strategies, we aim to dissect the functional role of adaptor-based membrane scaffolding proteins in TGN nanodomain organization to segregate and sort distinct membrane cargoes in polarized epithelial cells.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 958: Scaffolding of Membranes: Molecular Mechanisms and Cellular Functions
• Applicant Institution: Freie Universität Berlin
• Project Head: Professorin Francesca Bottanelli, Ph.D.