Spatial and temporal cellular assembly of the glycopeptide biosynthetic complex (B01+)

• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2019 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 398967434

Balhimycin, a glycopeptide synthesized by Amycolatopsis balhimycina, is a heptapeptide, which is modified by cyclization, methylation, halogenation, and glycosylation. Using genetics and biochemistry, its biosynthetic steps have been elucidated indicating the occurrence of a large multi-enzyme complex that catalyzes amino acid assembly as well as various modifications. Whereas the chronology of the reactions could be defined, nothing is known about the positioning and dynamics of such a super-complex. Protein interaction studies and state-of-the-art microscopy will localize the complexes in the cell, identify further putative interaction partners, and define the temporal assembly of the complex.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 261: Cellular Mechanisms of Antibiotic Action and Production
• Applicant Institution: Eberhard Karls Universität Tübingen
• Project Heads: Privatdozentin Efthimia Stegmann, Ph.D.; Professor Dr. Wolfgang Wohlleben