Targeting renoprotection downstream of the angiotensin II type 1 receptor (A05)

• Subject Area: Anatomy and Physiology ; Nephrology
• Term: from 2019 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 394046635

By modulating TRPC-mediated Ca2+ signaling in pre-glomerular cells, we aim to achieve renoprotection that is more specific than blockade of GPCRs such as AT1R. Using high-resolution particle analysis, we will characterize the general intracellular Ca2+ signals in the pre-glomerular region of a kidney slice model (Cx40 GCaMP mouse with a genetically encoded Ca2+ sensor in Cx40-expressing cells). Specific TRPC5/C6-mediated Ca2+ signals will be studied using knock-out mice and novel specific inhibitors. Based on an altered expression profile of TRPC channels in mouse models of metabolic syndrome (NZO) and subacute chronic renal failure (UUO), we plan a detailed characterization of the (modified) Ca2+ signals in these mouse models.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1365: Renoprotection
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Project Heads: Professor Dr. Maik Gollasch; Dr. Johanna Schleifenbaum