Mechanism of sphingolipid export from the endo-lysosomal system (P20*)

• Subject Area: Cell Biology
• Term: from 2019 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 180879236

Project P20 investigates how sphingolipids (SL) are transported in the endolysosomal system and how sphingolipid homeostasis is maintained. Although SLs are enriched at the plasma membrane they also play important roles in other organelles, such as the Golgi apparatus or vacuoles in yeast. The Golgilocalized GARP complex has an essential role in this process. To address how sphingolipids are sorted into and out of the yeast vacuole, the group will (i) determine the vacuolar sphingolipid content, (ii) identify vacuolar sphingolipid binding/transport proteins, and (iii) uncover the molecular mechanisms of vacuolar sphingolipid binding/transport proteins.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 944: Physiology and Dynamics of Cellular Microcompartments
• Major Instrumentation: Tracker S tensiometer
• Instrumentation Group: 1650 Oberflächen-Spannungsmesser
• Applicant Institution: Universität Osnabrück
• Project Head: Professor Dr. Florian Fröhlich