Project Details
Projekt
Infection free and scalable production of DNA oligonucleotides for mass production of nano-objects by self-assembling
Subject Area
Biological Process Engineering
Term
from 2018 to 2023
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 405686451
Studies in living organisms are the next milestone on the way to broad applicability of DNA-molecules for nanotechnology. For the latter as well as for studies on the application of DNA nano-objects in material sciences, the scalable supply of single-stranded-DNA (ss-DNA) will be necessary on a kilogram scale. Thus new ss-DNA production processes are required to make the step forward from gram to kilogram scale and even further. To enable the efficient biotechnological mass production of defined ss-DNA objects, it is essential to make use of existing biotechnological production plants. Using the infectious bacteriophage M13 for ss-DNA scaffold production or using a helperphage in combination with a phagemid for the production of user-defined and artificial ss-DNA with Escherichia coli is impossible with contract manufacturers, as the risk of unwanted phage infections persists throughout all other production processes performed there with E. coli. Thus the aim of this project is the rational design of a new process for infection free and efficient production of user-defined ss-DNA for self-assembly of nano-objects in a single-pot reaction. For this, instead of an infectious helperphage, a helperplasmid in combination with a phagemid is supposed to be used in E. coli.
DFG Programme
Research Grants
