Project Details
Projekt
Hybridization kinetics and non-equilibrium dynamics of random pools of oligonucleotides (P15)
Subject Area
Statistical Physics, Nonlinear Dynamics, Complex Systems, Soft and Fluid Matter, Biological Physics
Term
from 2018 to 2023
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 364653263
We will study, both experimentally and theoretically, hybridization kinetics within oligonucleotide pools and investigate how the composition of the pool – single-strands, partially hybridized, non-cognate complexes, and “perfectly matched” complexes – changes over time. As hybridization is strongly dependent on environmental parameters such as salt concentration and pH, we will then study the spatiotemporal dynamics of the sequence pools within salt gradients. We will develop experimental methods to monitor different species within the pool at different locations, and theoretical tools to analyze these complex dynamics.
DFG Programme
CRC/Transregios
Applicant Institution
Ludwig-Maximilians-Universität München
Project Heads
Professor Dr. Ulrich Gerland; Professor Dr. Friedrich Simmel
