Biochemical and functional analysis of P2X7 signaling (A15)

• Subject Area: Immunology
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 335447717

Activation of the pro-inflammatory P2X7 receptor induces diverse cellular responses. Using in situ cross-linking and mass spectrometry we have identified a range of potential interactors. We now aim to use our P2X7-EGFP mouse model in combination with biochemical and biophysical approaches to (i) define the role of P2X7 in cell migration and validate further interaction partners, (ii) elucidate cell type-specific P2X7 signaling pathways, (iii) determine the respective protein interaction sites and dynamics, and (iv) identify the binding sites and mode of action of P2X7-specific modulatory nanobodies.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1328: Adenine Nucleotides in Immunity and Inflammation
• Applicant Institution: Universität Hamburg
• Project Head: Professorin Dr. Annette Nicke