Synthesis, structural studies and characterization of ATP-hydrolyzing ectonucleotidase modulators (A11)

• Subject Area: Pharmacology
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 335447717

This project is aimed at developing potent, selective modulators of the ATP-hydrolyzing ecto-enzymes CD39 and NPP1. The extracellular domain of the human CD39, recombinantly expressed in Sf9 insect cells, will be co-crystallized with modulators that were developed in the first funding period. Structural information will allow further, targeted optimization of ortho- and allosteric modulators. Fluorophore-labeled modulators and photoactivatable prodrugs will be synthesized as biological tools. Selected modulators and labeled compounds will be synthesized in large scale to allow in vitro and in vivo studies by collaboration partners in models of inflammation and immunity.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1328: Adenine Nucleotides in Immunity and Inflammation
• Applicant Institution: Universität Hamburg
• Project Head: Professorin Dr. Christa E. Müller