Dynamic architecture of Ca2+ microdomains in T cells: disentangling contribution of adenine nucleotides, Ca2+ release and Ca2+ entry (A02)

• Subject Area: Immunology
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 335447717

Activation of T cells is closely related to the occurrence of Ca2+ microdomains. Our project aims to unravel the interplay of the complementary players during early T cell activation. Here, we focus on the formation of antigen dependent Ca2+ micro- and even nanodomains, the characterization of ‘pure’ NAADP evoked Ca2+ signals as well as the visualization of the initial ATP concentration surrounding single T cells. To resolve and analyze associated Ca2+ micro-/nanodomains, the project develops and integrates innovative artificial intelligence and image processing algorithms and high-speed super-resolution live cell imaging.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1328: Adenine Nucleotides in Immunity and Inflammation
• Applicant Institution: Universität Hamburg
• Project Heads: Dr. Bjoern-Philipp Diercks, since 1/2020; Professor Dr. René Werner; Dr. Insa Wolf, until 12/2019