Evaluating anti-oxidant and autophagy compounds as potential therapies for mammalian aminoglycoside-induced hair cell loss

• Applicant: Dr. Clara Sophia Draf
• Subject Area: Otolaryngology, Phoniatrics and Audiology
• Term: from 2017 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 392120974

• Final Report Year: 2021

Final Report Abstract

Conclusion. The preponderance of the results from our screen of an autophagy library suggest that autophagy contributes to ototoxic HC damage. However, the compounds tested have many potential alternative effects. For this reason we cannot conclude with confidence that changes in autophagy were the primary reason for HC protection. Additional studies will need to be performed to further validate the results of our screen and to determine the relationship between autophagy and HC damage.

Publications

• 90th Annual Meeting of the German Society of Oto-Rhino-Laryngology 2019: Die Rolle von Autophagieverbindungen im Hinblick auf Ototoxizität. Eine in-vitro-Screening Studie
  Draf C, Wyrick T, Chavez E, Pak K, Dazert S, Ryan AF
  
• the 42nd Meeting of the Association for Research in Otolaryngology 2019: Evaluating Autophagy Compounds as Potential Therapies for Mammalian Aminoglycoside-Induced Hair Cell Loss
  Draf C, Wyrick T, Chavez E, Pak K, Dazert S, Ryan AF
  
• Single-Cell Transcriptomes Reveal a Complex Cellular Landscape in the Middle Ear and Differential Capacities for Acute Response to Infection. Front Genet. 2020
  Ryan AF, Nasamran C, Pak K, Draf C, Fisch K, Webster N, Kurabi A
  (See online at https://doi.org/10.3389/fgene.2020.00358)
• 44th Meeting of the Association for Research in Otolaryngology 2021: An in vivo Model as an Approach to Deliver Potential Therapies for Noise-Induced Hair Cell Loss
  Draf C, Chavez E, Kwang P, Leichtle A, Kurabi A, Dazert S, Ryan AF