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Mechanisms of pNaSS-grafted poly(epsilon)-caprolactone scaffolds for enhanced rAAV-mediated gene transfer in human bone marrow-derived mesenchymal stem cells

Subject Area Orthopaedics, Traumatology, Reconstructive Surgery
Term from 2017 to 2020
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 388654311
 
Final Report Year 2020

Final Report Abstract

During the DFG Fellowship "Mechanisms of pNaSS-grafted poly(epsilon)-caprolactone scaffolds for enhanced rAAV-mediated gene transfer in human mesenchymal stem cells", the applicant Dr. Jagadeesh K. Venkatesan and his supervisors Profs. Magali Cucchiarini and Henning Madry were able to bring evidence of the suitability of the use of pNaSS-grafted PCL scaffolds as new efficient delivery systems of therapeutic (TGF-β, SOX9) rAAV vectors as an improved means to durably and safely modify human bone marrow aspirates relative to free vector treatments with enhanced chondrogenic features relevant of the processes of cartilage repair. This fact highlights the potential of combining adapting tissue engineering platforms to gene transfer approaches mediated by rAAV vectors as an attractive tool to circumvent the natural obstacles from these clinically adapted vectors to achieve an efficient and durable gene expression of the therapeutic sequences within the lesions.

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