Metabolic impact on chromatin dynamics - understanding control of gene expression through metabolic enzymes and their products (A20)

• Subject Area: Biochemistry
• Term: since 2017
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

Most chromatin modifiers require co-factors that are metabolic intermediates. To understand mechanistically the cross-talk between metabolism and chromatin we will (i) manipulate selected metabolic enzymes that produce or convert co-factors to unravel their impact on histone PTMs locally and globally (ii) recruit metabolic enzymes to genes to reveal - in combination with in vitro reconstitutions - their effect on transcription and (iii) identify (nuclear) interactions between histone modifiers and metabolic enzymes as well as their genomic location. This will give us important insights in how metabolic enzymes impact histone PTMs and how chromatin responds to metabolic challenges.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professor Dr. Robert Schneider