AC-electrostimulation of cells on functionalised and laterally structured material surfaces – cell signalling aspects (A03)

• Subject Area: Biomaterials ; Experimental Condensed Matter Physics
• Term: since 2017
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 299150580

In this project, two cell signalling pathways – Ca2+ and Wnt – under alternating current stimulation in vitro are in focus. The intracellular calcium ion mobilisation in vital human osteoblasts and mesenchymal stem cells will be locally recorded via scanning ion conductance microscopy and confocal laser scanning microscopy. In vitro data of components of the Wnt signalling pathway and of reactive oxygen species will be delivered for modelling and simulation in silico. A decisive step is the preparation of electrode patterns to be covered by polymers. The potential at the electrodes will be independently controlled allowing for the assessment of lamellipodia formation or membrane ruffle alterations towards specific electric signals or patterns.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1270: ELectrically Active ImplaNts - ELAINE
• Applicant Institution: Universität Rostock
• Project Heads: Professorin Dr. J. Barbara Nebe; Dr. Henrike Rebl, since 7/2021; Professorin Dr. Sylvia Speller