Modules involved in endosomal/lysosomal Ion homeostasis and function (C05)

• Subject Area: Biochemistry
• Term: from 2007 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

Results from our studies focusing on vesicular CLC anion/proton exchangers that were predicted to provide an electrical shunt for the H+-ATPase (responsible for vesicle acidification), indicate much more complex and diverse roles of transporters in this functional module. We therefore aim at analyzing many different vesicular transporters in combination by use of sophisticated mouse models and cell culture systems applying newly available techniques (CRISPR-Cas system). Moreover the role of ClC 7 (lysosomal anion transporter)/kinesin-2-interactions in exocytosis of lysosomes will be investigated.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Co-Applicant Institution: Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP); im Forschungsverbund Berlin e.V.; Max-Delbrück-Centrum für Molekulare Medizin (MDC)
• Project Head: Professor Dr. Thomas J. Jentsch