In vitro and clinical comparison of corneal grafts cultured in serum free medium or standard serum supplemented medium in patients with degeneration of the corneal endothelium

• Applicant: Professorin Dr. Katrin Engelmann
• Subject Area: Ophthalmology
• Term: from 2007 to 2013
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 34896408

• Final Report Year: 2013

Final Report Abstract

No abstract available

Publications

• (2001) Effect of three different media on organ-cultured donor corneas and Isolated human corneal endothelial cells. Br J Ophthalmol 85: 1416-1420
  Bednarz J, Doubilei V, Wollnik PCM, Engelmann K
  
• (2001) Evaluation of potential organ culture media for eye banking using a human corneal endothelial cell growth assay. Graefe's Arch Clin Exp Ophthalmol 239: 778-782
  Møller-Pedersen T, Hartmann U, Ehlers N, Engelmann K
  
• (2001) Evaluation of potential organ culture media for eye banking using human donor corneas. Br J Ophthalmol 85: 1075-1079
  Møller-Pedersen T, Hartmann U, Møller HJ, Ehlers N, Engelmann K
  
• (2001) Use of a serum-free medium for long-term storage of human corneas. Influence on endothelial cell density and corneal metabolism. Graefe's Arch Clin Exp Ophthalmol 239: 801-805
  Hempel B, Bednarz J, Engelmann K
  
• (2004) Prospects for endothelial transplantation. Exp Eye Res 78: 573-578
  Engelmann K, Bednarz J, Valtink M
  
• (2007) Serum-free cultivation of adult normal human choroidal melanocytes. Graefe's Arch Clin Exp Ophthalmol 245:1487-1494
  Valtink M, Engelmann K
  
• (2008) Cultivation of an immortalized human corneal endothelial cell population and two distinct clonal subpopulations on thermo-responsive carriers. Graefe's Arch Clin Exp Ophthalmol. 246:1575-1583
  Götze T, Valtink M, Nitschke M, Gramm S, Hanke T, Engelmann K, Werner C
  
• (2010) Alignment And Cell-Matrix Interactions Of Human Corneal Endothelial Cells On Nanostructured Collagen Type I Matrices. Invest Ophthalmol Vis Sci 51:6303-6310
  Gruschwitz R, Friedrichs J, Valtink M, Franz C, Müller DJ, Funk RHW, Engelmann K
  
• (2010) Screening zur Qualitätsbestimmung von Medien und Medienbestandteilen für die Kultivierung von Spenderhornhäuten zur Hornhauttransplantation. Reg Med 2/2010:49-53
  Engelmann K, Valtink M, Knels L, Funk RHW, Knipper A
  
• (2011) Retroviral transduction of human corneal endothelial cells: Implication of pseudotyping on transduction efficiency and viral cytotoxicity. Invest Ophthalmol Vis Sci 52(9):6807-13
  Valtink M, Stanke N, Knels L. Engelmann K, Funk RHW, Lindemann D
  
• (2011) Serum-free SFM corneal organ culture medium but not conventional MEM organ culture medium protects human corneal endothelial cells from apoptotic cell death. Br J Ophthalmol 95:123-130
  Jäckel T. Valtink M, Knels L, Funk RHW, Engelmann K
  
• (2012) Overexpression of human HMW FGF-2 but not LMW FGF-2 reduces the cytotoxicity of lentoviral gene transfer in human corneal endothelial cells. Invest Ophthlmol Vis Scl 31:3207-14
  Valtink M, Knels L, Stanke N, Engelmann K, Funk R, Lindemann D