Transcriptional networks regulating multiple drug resistance (MDR) in Candida albicans

• Applicant: Professor Dr. Joachim F. Ernst
• Subject Area: Parasitology and Biology of Tropical Infectious Disease Pathogens
• Term: from 2006 to 2011
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 34405591

• Final Report Year: 2011

Final Report Abstract

Candida albicans ist der bedeutendste Erreger von ernsten Pilzerkrankungen des Menschen. In einem deutsch-indischen Gemeinschaftsprojekt gefördert durch DFG und DST wurde die Regulation von Genen untersucht, die für die Resistenz von C. albicans notwendig sind. In der Arbeitsgruppe Düsseldorf wurde die Expression der fünf PMT-Gene analysiert, die die O-Mannosylierung sekretorischer Proteine und gleichzeitig Antimykotika-Resistenz bewirken. Für die Expression des PMT1 ergab sich unter Stressbedingungen eine Regulation auf der Ebene der Transkription und dafür erforderliche Promotorsequenzen wurden identifiziert. Für die Expression der PMT2- und PMT4-Gene ergab sich demgegenüber ein posttranskriptioneller Regulationsmechanismus.

Publications

• (2009) Molecular mechanisms for negative autoregulation of the EFG1 gene in Candida albicans. 27th Small Meeting on Yeast Transport and Energetics. Blankenberge, Belgien, 4.-8.9.09
  Lassak, T. and Ernst, J. F.
  
• (2009) Morphogenic regulator Efg1p of Candida albicans affects drug susceptibilities independent of drug efflux pumps. 3rd FEBS Advanced Lecture Course "Human Fungal Pathogens", La Colle sur Loup, France, 2.-8.5.09
  Prasad, T., Hameed, S., Mukhopadhyay, C. K., Biswas, S., Setiadi, E. R., Ernst, J. F. and Prasad, R.
  
• (2009) Morphogenic regulator EFG1p of Candida albicans affects drug susceptibilities independent of drug efflux pumps. 6th Annual Research festival "BIOSPARKS 2008", School of Life Sciences, JNU, New Delhi
  Prasad, T., Hameed, S., Manoharlal, R., Setiadi, E. R., Cottier, F., Mukhopadhyay, C. K., Ernst, J. F. and Prasad, R.
  
• (2009) Morphogenic regulator EFG1p of Candida albicans affects drug susceptibilities independent of drug efflux pumps. Third FEBS Advanced lecture Course, Human Fungal Pathogens: Molecular Mechanisms of Host Pathogen Interaction and Virulence, La Colle Sur Loup, France, 2nd - 5th May
  Prasad, T., Hameed, S., Mukhopadhyay, C. K., Biswas, S., Manoharlal, R., Setiadi, E. R., Ernst, J. F, and Prasad, R.
  
• (2009) Regulation of PMTgenes encoding protein O-mannosyltransferases in Candida albicans. 3rd FEBS Advanced Lecture Course "Human Fungal Pathogens", La Colle sur Loup, France, 2.-8.5.09
  Cantero, P. D. and Ernst, J. F.
  
• (2009) Regulation of the PMT1 gene in Candida albicans. IV International Conference on Molecular Mechanisms in Fungal Cell Wall Biogenesis. Warshaw, Poland, 3.8.-3.9.09
  Cantero, P. and Ernst, J. F.
  
• (2010) Morphogenic Regulator EFG1 Affects Drug Susceptibilities of Pathogenic Candida albicans. 10th ASM conference on Candida and Candidiasis, Miami, USA, 22.-26.3.10
  Hameed, S., Prasad, T., Manoharlal, R. and Prasad, R.
  
• (2010) Morphogenic regulator EFG1 affects drug susceptibilities of pathogenic Candida albicans. FEMS Yeast Res. 10:587-596
  Prasad, T., Hameed, S., Manoharlal, R., Biswas, S., Mukhopadhyay, C. K., Goswami, S. K. and Prasad, R.
  
• (2010) O-mannosylation and signalling pathways in Candida albicans. 10th ASM conference on Candida and Candidiasis, Miami, USA, 22.-26.3.10
  Cantero, P. D. and Ernst, J. F.
  
• (2011) Damage to the glycoshield activates PMT-directed O-mannosylation via the Msb2-Cek1 pathway in Candida albicans. Mol. Microbiol.
  Cantero, P. D. and Ernst, J. F.
  (See online at https://doi.org/10.1111/j.1365-2958.2011.07604.x)