Dynamics of functional membrane complexes associated with the plasma membrane H+-ATPase AHA2

• Applicants: Professor Dr. Klaus Harter; Professorin Dr. Waltraud Schulze
• Subject Area: Plant Physiology ; Plant Biochemistry and Biophysics
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 327797394

• Final Report Year: 2021

Final Report Abstract

In conclusion, we here present a strategy involving path-based centrality measures to describe dynamic rewiring of cellular protein-protein interaction networks in response to external stimuli. This approach identified AT5G49770 as a novel player in nitrate-induced responses, which was corroborated by phenotypic analysis. We expect the use of path-based centrality measures to be a powerful strategy also in the analysis of dynamic responses of cellular networks to other stresses and stimuli. Furthermore, we were able to implement several techniques such as three-fluorophor intensity-based FRET and FRET-FILM as well as PALM-based SRM methods in living plant cells.

Publications

• (2017) Highly Efficient Single-Step Enrichment of Low Abundance Phosphopeptides from Plant Membrane Preparations. Frontiers in Plant Science, 8: 1673
  Wu XN, Xi L, Pertl-Obermeyer H, Li Z, Chu LC, Schulze WX
  (See online at https://doi.org/10.3389/fpls.2017.01673)
• (2019) Global Identification of Protein Complexes within the Membrane Proteome of Arabidopsis Roots Using a SEC-MS Approach. Journal of Proteome Research, 18: 107-119
  Gilbert M, Schulze WX
  (See online at https://doi.org/10.1021/acs.jproteome.8b00382)
• (2019) Quantitative description and computational modelling of the BRI1 response module controlling root cell elongation growth – from organ-scale to nanodoamins. Doctoral Degree (rer. nat.)
  Glöckner, Nina
  (See online at https://doi.org/10.15496/publikation-35158)
• (2021) A modular, comprehensive microscopy platform for modern live cell imaging. Doctoral Degree (rer. nat.)
  Zur Oven-Krockhaus, Sven
  (See online at https://doi.org/10.15496/publikation-62250)
• (2021) Comparison of path-based centrality measures in protein-protein interaction networks revealed proteins with phenotypic relevance during adaptation to changing nitrogen environments. Journal of Proteomics, PMID: 33453437
  Gilbert M, Li Z, Wu XN, Rohr L, Gombos S, Harter K, Schulze WX
  (See online at https://doi.org/10.1016/j.jprot.2021.104114)
• (2021) Prediction of protein-protein complexes by combining size exclusion chromatography and mass spectrometric analysis. Doctoral Degree (rer. nat.)
  Gilbert, Max
  
• (2021) Three-fluorophore FRET-FLIM enables the study of trimeric protein interactions and complex formation with nanoscale resolution in living plant cells
  Glöckner N, Zur Oven-Krockhaus S, Wackenhut F, Burmeister M, Wanke F, Holzwart E, Wolf S, Harter K
  (See online at https://doi.org/10.1101/722124)