Project Details
Projekt
Neurotransmitter release: spatio-temporal characterization of membrane fusion intermediates (A04)
Subject Area
Molecular Biology and Physiology of Neurons and Glial Cells
Term
since 2016
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 278001972
Project A04 examines members of two functionally distinct classes of switch modulators of neurotransmitter release (Complexin 1/2 vs -3/4 and Doc2a vs. Doc2b) at the level of in vitro reconstitution of docking/fusion assays (Söllner) and in native synapses using synaptic transmission analysis (Rosenmund). A detailed characterization of the molecular features of the distinct isoforms by biochemical and electrophysiological methods will provide new insights into the mechanism and dynamics of release modulators - the functional properties of their domains and their distinct molecular rearrangements at different stages of the membrane fusion process.
DFG Programme
CRC/Transregios
Applicant Institution
Freie Universität Berlin
Project Heads
Professor Dr. Christian Rosenmund; Professor Dr. Thomas Söllner
