High resolution analysis of active zone cytomatrix remodeling (A03)

• Subject Area: Molecular Biology and Physiology of Neurons and Glial Cells
• Term: since 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 278001972

Project A03 focuses at obtaining a deeper understanding of the processes involved in presynaptic active zone (AZ) remodeling, which control the release of synaptic vesicles. To this end we will employ simulated emission depletion (STED) and single molecule super-resolution fluorescence microscopy, as well as electron microscopy (EM) and cryogenic electron tomography (cryo-ET) to analyze conserved cytomatrix proteins architectures. Ultimately, we aim to arrive at a description of the AZs macromolecular scaffold organization and its dynamics during adaption in the (sub-)nanometer range.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 186: Molecular Switches: Spatio-temporal Control of Cellular Signal Transmission
• International Connection: Denmark
• Applicant Institution: Freie Universität Berlin
• Project Heads: Professorin Dr. Cristina Paulino, since 7/2024; Professor Dr. Carsten Schultz, until 9/2019; Professor Dr. Stephan J. Sigrist; Dr. Alexander Walter, until 6/2024