Mitochondrial structure and dynamics in light and electron microscopy (Z03)

• Subject Area: Cell Biology
• Term: since 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 269925409

We will move towards an increased automation in EM and 3D EM image acquisition by implementing a published Python module in SerialEM. This will allow us to identify and acquire cells of interest more efficiently, especially in serial sectioning approaches. In collaboration with the García-Sáez lab we will further improve on mitochondria recognition for LM images using AI and deep-learning algorithms. We will adapt these workflows to identify and segment mitochondria in EM images of cells and tissue. For this, trainings data sets will be acquired and annotated. In addition, we will improve our CLEM and Super-CLEM protocols to the needs of the CRC and combine it with high-pressure freezing sample preparation to prevent fixation artefacts in mitochondrial ultrastructure.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1218: Mitochondrial regulation of cellular function
• Applicant Institution: Universität zu Köln
• Project Heads: Professorin Dr. Ana Jesús Garcia Sáez, from 7/2020 until 6/2024; Dr. Astrid Schauss