Project Details
Projekt
Nanoimaging of DNA nanoagents interacting with cells (A11)
Subject Area
Biochemistry
Term
from 2016 to 2024
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 201269156
Many fundamental processes in biology happen at interfaces. Especially the plasma membrane is of major interest for cell biological and biophysical studies, as it links the inner workings of cells to their surroundings and allows them to sense, interact, and react. We plan to develop methodologies using DNA-PAINT and DNA origami nanoagents to probe and manipulate cell surface receptor molecules on the level of single proteins with thus far unprecedented detail and precision in control. We will use novel labeling probes to probe a multitude of cell surface receptors on the single protein level, followed by prescribed perturbation using DNA origami structures. We also aim to elucidate the inner workings of the focal adhesome by probing and manipulating key players in adhesion using DNA-PAINT and DNA-based nanoagents.
DFG Programme
Collaborative Research Centres
Applicant Institution
Ludwig-Maximilians-Universität München
Project Head
Professor Dr. Ralf Jungmann
