Dynamics of proteins of the inner nuclear membrane (P07)

• Subject Area: Biochemistry
• Term: from 2016 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 264061860

The nucleus is surrounded by the inner and the outer nuclear membrane (INM, ONM), which are connected at the level of the nuclear pores, the gates between the nucleus and the cytoplasm. In this project, we want to investigate INM-proteins, with one focus on VAPB, a protein that has previously been described as an ER-resident protein found in many contact sites. We investigate the functional relevance of novel VAPB-interactions with proteins at the INM and the nuclear pore complex that we identified using a novel, proximity-based screening approach. Furthermore, we plan to analyse the dynamics of VAPB (and other INM-proteins) by life-cell microscopy and to investigate a mutant form of VAPB that is involved in a familial form of amyotrophic lateral sclerosis.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1190: Compartmental Gates and Contact Sites in Cells
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Ralph Kehlenbach