MAIRA - Hoch effiziente und genaue Algorithmen für die mobile Analyse von Mikroben

• Antragsteller: Professor Dr. Daniel H. Huson
• Fachliche Zuordnung: Bioinformatik und Theoretische Biologie ; Parasitologie und Biologie der Erreger tropischer Infektionskrankheiten
• Förderung: Förderung von 2015 bis 2021
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 289436404

• Erstellungsjahr: 2021

Zusammenfassung der Projektergebnisse

The aim of this project was to develop new algorithms and software to support real-time analysis of mobile sequencing technologies. One main result of this work is an improved understanding of the challenges specific to the analysis of metagenomic long reads, such as the problem of frame shifts caused by sequencing errors. This has led to the development of new algorithms to address these challenges and the extension of the DIAMOND+MEGAN metagenomic analysis pipeline so as to accommodate long reads. A second main result is the development of the new MAIRA program, which runs on a laptop and processes the output of a MinION sequencing in real-time. We are currently exploring the use of MAIRA to monitor the dynamics of microbial communities in bioreactors. In future, the software will be extended to applications in genome mining. Moreover, we intend to deploy the software for in-field DNA analysis in situations in which access computational infrastructure is difficult.

Projektbezogene Publikationen (Auswahl)

• (2018). MEGAN-LR: New algorithms allow accurate binning and easy interactive exploration of metagenomic long reads and contigs. Biology Direct, 13(6)
  DH Huson, B Albrecht, C Bagci, I Bessarab, A Gorska, D Jolic and RBH Williams
  
• (2019). Annotated bacterial chromosomes from frame-shift-corrected long read metagenomic data. Microbiome, 7(61)
  K Arumugam, C Bagci, I Bessarab, S Beier, B Buchfink, A Gorska, G Qiu, DH Huson and RBH Williams
  
• Introduction to the analysis of environmental sequences: Metagenomics with MEGAN, Evolutionary Genomics, Humana, New York, NY, 591-604 (2019)
  C Bagci, S Beier, A Gorska, DH Huson
  
• (2020) MAIRA-real-time taxonomic and functional analysis of long reads on a laptop, BMC Bioinformatics 21 (13), 1-12
  B Albrecht, C Bagci, DH Huson
  
• (2021) DIAMOND+MEGAN: Fast and easy taxonomic and functional analysis of short and long microbiome sequences, Current Protocols, Volume 1, Issue 3
  C Bagci, S Patz, DH Huson
  
• (2021) The isolate Caproiciproducens sp. 7D4C2 produces n-caproate at mildly acidic conditions from hexoses: genome and rBOX comparison with related strains and chain-elongating bacteria. Frontiers in Microbiology, Vol. 11, No. 594524
  S Esquivel-Elizondo, C Bagci, M Temovska, B Seung Jeon, I Bessarab, RBH Williams, DH Huson, LT Angenent