Project Details
Projekt
Protein biogenesis and transport to the endoplasmic reticulum during health, aging and proteotoxic stress in Caenorhabditis elegans (A07*)
Subject Area
Biochemistry
Term
from 2016 to 2023
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 189682160
The project investigates the function of the ribosome-associated nascent polypeptide-associated complex (NAC) in protein biogenesis and transport. Recently, we have revealed that NAC contacts nascent polypeptide chains already deep within the ribosomal tunnel. We will address the question how the early nascent chain sensing inside the tunnel impacts co-translational protein biogenesis and transport pathways. Studies will be performed in vitro as well as in vivo using the animal model Caenorhabditis elegans. Furthermore, we recently found that NAC exerts broad-spectrum chaperone activities involving multiple substrate binding sites. We will perform a detailed analysis of the chaperone function of NAC aiming to identify all critical substrate binding sites of NAC and their modes of action involved in chaperoning co- and post-translational substrates.
DFG Programme
Collaborative Research Centres
Applicant Institution
Universität Konstanz
Project Heads
Professorin Dr. Elke Deuerling; Martin Gamerdinger, Ph.D.
