Serine ubiquitination and regulation of autophagy upon Legionella (E01 (C01))

• Subject Area: Biochemistry ; Biophysics ; Cell Biology
• Term: from 2016 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 259130777

We will examine the impact of serine ubiquitination on the host autophagic pathway and how this might affect bacterial infection by Legionella. We will perform functional studies to elucidate the contribution of Legionella SidE proteins and RavZ in impairing host autophagy. We will use super-resolution microscopy to study the nanoscale protein organization around the Legionella-containing vacuole, focusing on autophagy, signaling proteins, and ubiquitination. We will develop a functional, spatiotemporal and molecular picture of processes that guard Legionella from autophagy-mediated clearance.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1177: Molecular and Functional Characterization of Selective Autophagy
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Heads: Professor Dr. Ivan Dikic; Professor Dr. Mike Heilemann, since 1/2020