Project Details
Projekt
Role of the cell junction-assocaited mechanical forces in the regulation of organ size in zebrafish
Applicant
Professorin Dr. Virginie Lecaudey
Subject Area
Developmental Biology
Cell Biology
Cell Biology
Term
since 2015
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 273724439
Epithelia are tightly packed cell layers that surround and protect our organs. In these layers each epithelial cell is highly polarized and mechanically attached to several neighbors through characteristic intercellular junctions that connect their cytoskeleton. The actomyosin cytoskeleton associated with cell adhesion thus generates junctional tension and mechanical forces, which are required to maintain and organize these cells as a tissue. Although it is now well recognized that cell behaviors including cell proliferation and cell fate, results from the concerted action of biochemical and mechanical signals at cell-cell junctions, the molecular mechanisms allowing cells to sense mechanical forces and integrate them with biochemical signaling are only starting to be understood. As proteins that shuttle between the cell membrane and the nucleus, the Hippo signaling pathway effectors Yap and Taz and proteins of the Ajuba and Zyxin family, are perfect candidates to convert signals from the cell surface into transcriptional responses. Here we want to investigate in vivo how these two classes of families link tension at cell-cell junction to cell behavior and tissue morphogenesis. The lateral line (LL) of zebrafish has recently emerged as a very powerful model to study epithelial morphogenesis and collective migration. The lateral line primordium (LLP) is a group of about 100 cells that delaminate from an epithelial placode and collectively migrate as a group of adherent cells on both sides of the fish embryo. As they migrate, cells in the trailing region undergo apical constriction to form radially organized rosettes that are then deposited and differentiate into mechanosensory organs. We have shown that the junction-associated protein Shroom3 is required for apical constriction-mediated rosette assembly within the LLP. Furthermore, we have shown that the Motin protein Amotl2a is essential to control the size of the LLP by physically interacting with and inhibiting the Hippo effector Yap1. Recently, we have identified three proteins of the Ajuba/Zyxin family as interacting partners of Shroom3. Given that Ajuba/Zyxin proteins have recently been proposed to link junctional tension to Yap, they are good candidates to link morphogenesis to cell proliferation in the LLP. Therefore, the main goal of the proposed project is to investigate the role of the Shroom3 - Ajuba/Zyxin – Hippo axis in zebrafish in vivo. We want to understand how this complex could be involved in transmitting physical forces associated with changes in cell shape by linking cell junctions to the intracellular cytoskeleton and to Yap-mediated transcription to modulate proliferation.
DFG Programme
Priority Programmes
