Die Bedeutung von TRPC6 für die Podozytenfunktion und die Pathogenese der fokal segmentalen Glomerulosklerose

• Applicant: Professor Dr. Gerd Walz
• Subject Area: Nephrology
• Term: from 2006 to 2009
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 27112833

• Final Report Year: 2012

Final Report Abstract

Depletion of zebrafish TRPC6 or TRPC7 disrupts the normal glomerular filtration barrier, thereby resembling the human phenotype. Our results demonstrate that the zebrafish pronephros model represents a useful model system to study the nephrotic syndrome, a phenotypic manifestation of several acquired and hereditary glomerular diseases. Ongoing work needs to more precisely localize the gene products of TRPC6.1 and TRPC7.1 within the zebrafish glomerulus (i.e. demonstrate that these two genes are expressed in zebrafish podocytes), and analyze the structural defects caused by knockdown of these two genes at the ultrastructural level (i.e. by electron microscopy).