Project Details
Projekt
Cytocompatibility and bioactivity testing in vitro
Applicant
Dr. Birgit Weyand
Subject Area
Biomaterials
Term
from 2015 to 2020
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 251503496
Aim of this subproject is the development of a standardized cell-based assay method in or-der to test the suitability of new implant materials For the test procedure we will use mesenchymal stem cells in order to evaluate the biocompatibility of the material components. Furthermore, we will develop strategies to improve cell seeding and attachement, as well as static and dynamic culture techniques together with biomechanical stimulation of the cell-matrix-constructs in order to mimic physiological culture conditions.Both groups will isolate and characterise mesenchymal stem cells using identical protocols. In order to achieve sufficient cell numbers for testing, dynamic expansion techniques such as the Wave technology (cellbag bioreactors on mixing systems) will be established and optimized. Be-sides of microcarrier-based technologies we intend to use also particle-free microaggregate culture techniques. Besides we will study the influence of hypoxia (5% O2) on proliferation, differentiation and senescence of mesenchymal stem cells.Furthermore we will work on strategies to optimize cell seeding and attachment on different materials. Beside of cellular attachment to surface properties we will study the cellular ingrowth in-side the matrices. The cell-seeded constructs will then be cultured under dynamic conditions in specialized bioreactor systems and differentiated into the particular tissue types, such as tendon or bone, for functional testing of the implant. In this part of the project we will concentrate on protein-functionalized matrices from TP1, TP2, and TP5 seeded with mesenchymal stem cells and we will test the effects of proteins on cell differentiation. In parallel, we will test impact and efficiency of different sterilization methods in static long-term cultures.
DFG Programme
Research Units
International Connection
Austria
Partner Organisation
Fonds zur Förderung der wissenschaftlichen Forschung (FWF)
Cooperation Partner
Professorin Dr. Cornelia Kasper
