pH in the TGN/EE as specificity determinant in the post-Golgi trafficking (A02)

• Subject Area: Plant Cell and Developmental Biology ; Bioinformatics and Theoretical Biology
• Term: since 2014
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 232631280

The TGN/EE is a highly dynamic compartment through which both newly synthesized and endocytosed proteins have to pass. Acidification of the TGN/EE by the V-ATPase is an important determinant of compartmental identity and we will elucidate the molecular mechanism that targets a specific isoform of the V-ATPase to the TGN/EE where it can act as a pH-sensor that directly interacts with components of the trafficking machinery. Super-resolution microscopy will be used to identify functionals subdomains of the TGN/EE and to correlate them with their luminal pH. Based on the data obtained, our mathematical model of pH-homeostasis in the TGN/EE will be refined and tested.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1101: Molecular Encoding of Specificity in Plant Processes
• Applicant Institution: Eberhard Karls Universität Tübingen
• Project Heads: Professorin Dr. Ursula Kummer; Professorin Dr. Karin Schumacher