Targeting centromere recruitment of mitotic regulators by supramolecular ligands (B06)

• Subject Area: Cell Biology
• Term: from 2014 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 229838028

Kinetochores are multi-subunit protein assemblies that mediate chromosome segregation during cell division. In this proposal, we will build on our recent in vitro biochemical reconstitution of kinetochores and of their regulation. We will explore the potential of supramolecular compounds generated within the CRC to modulate reversible protein-protein interactions, with a special focus on those mediated by phosphorylation. For this, we plan to use a variety of biochemical assays, including real-time assays that monitor reaction kinetics, which we will use to assess perturbations introduced by supra-molecular ligands.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1093: Supramolecular Chemistry on Proteins
• Applicant Institution: Universität Duisburg-Essen
• Project Head: Professor Dr. Andrea Musacchio