DNA origami nanoarchitectures for predesigned spatial confinement of proteins (A06)

• Subject Area: Biological and Biomimetic Chemistry
• Term: from 2014 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 229838028

In this project we aim at developing a general host-guest complexation strategy for the manipulation of the stability and activity of desired proteins specifically encapsulated into customized DNA cages. To this end, we will use a large tool of supramolecular ligands with distinct specificity, affinity and associated read-out signals. Spatial control of multivalent ligand-protein interactions will be also employed to mimic the architecture of fundamental components of the kinetochore-microtubule interface, thus enabling to gain a deeper insight into the mechanisms of chromosome segregation and more in general to offer a strategy for the generation of artificial protein machineries. 

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1093: Supramolecular Chemistry on Proteins
• Applicant Institution: Universität Duisburg-Essen
• Project Head: Professorin Dr. Barbara Saccà