Role of the BEACH domain protein SPIRRIG in the context of the ESCRT-System in Arabidopsis thaliana
Evolutionary Cell and Developmental Biology (Zoology)
Final Report Abstract
This proposal aimed to explore the function of the SPI protein in the ESCRT endosomal sorting system in Arabidopsis thaliana. During the course of the experiments we developed four project parts. In the first part we demonstrate that SPI is involved in the regulation pathway of LIP5 and SKD1 in the context of vacuolar trafficking. In the second part we studied the function of SPI in Arabis alpina. We show that Arabis spi mutants share some but not all phenotypes with Arabidopsis indicating a functional diversification. To study the protein interactions in vivo, we performed co-immunopreciptiation assays with SKD-YFP and determined the interactome by mass-spectrometry. In the forth part, we identified RabE1c as a repressor of the spi mutant morphogenesis phenotype suggesting that changes in the secretion pathway may compensate the absence of SPI in vacuolar trafficking.
Publications
- 2015. The BEACH Domain Protein SPIRRIG Is Essential for Arabidopsis Salt Stress Tolerance and Functions as a Regulator of Transcript Stabilization and Localization. PLoS Biol 13(7): e1002188
Steffens A, Brautigam A, Jakoby M, Hulskamp M
(See online at https://doi.org/10.1371/journal.pbio.1002188) - 2017. Physical, Functional and Genetic Interactions between the BEACH Domain Protein SPIRRIG and LIP5 and SKD1 and Its Role in Endosomal Trafficking to the Vacuole in Arabidopsis. Frontiers in Plant Science 8
Steffens A, Jakoby M, Hulskamp M
(See online at https://doi.org/10.3389/fpls.2017.01969)
