EXC 114:  Munich Center for Integrated Protein Science (CIPSM)

• Subject Area: Basic Research in Biology and Medicine
• Term: from 2006 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 24286268

• Final Report Year: 2019

Final Report Abstract

Proteins are the principal carriers of function in each living cell and of central importance in life. At the beginning of the cluster it became apparent that proteins will be the next generation pharmaceuticals. Indeed today, proteins have revolutionized the way in which we treat cancer. The goal of the excellence cluster CIPSM was to chaperone these new developments. The CIPSM cluster established towards this goal six research areas. In the first research area, protein biophysics, CIPSM studied the stability of proteins and the forces that shape the protein universe. In the second research area CIPSM investigated the principles of proteins folding and misfolding. The third research area was devoted to study the three-dimensional structure of proteins with X-ray crystallography, cryo-electron microscopy and NMR spectroscopy. The fourth research area bundled research in the area of protein nucleic acids interactions, recognizing that proteins are intimately involved in the regulation of transcription. In the fifth research area of CIPSM, new chemical biology and protein engineering tools were developed to manipulate proteins. In research area six, CIPSM investigated proteins involved in diseases with particular emphasis on developing photo-pharmacological approaches, and protein function in systems. Systems biology was newly established in Munich by CIPSM. In all six research areas, CIPSM was able to achieve major scientific breakthroughs as demonstrated by over 2400 publications, among them 25 publications in Nature, 27 publications in Science and 15 publications in Cell. CIPSM also successfully moved scientific results into applications. The elucidation of the silk protein structure and the development of efficient expression systems led not only to high impact publications but allowed the creation of the company AMSilk GmbH. New click chemistry methods were developed to manipulate biomolecules, which delivered not only highly cited publications but led also to the establishment of the company Baseclick GmbH which now provides technologies for next generation sequencing. The CIPSM effort to create highly potent antibodies led to the development of camel-bodies and the spin-outs ChromoTek GmbH and Tubulis GmbH. New ideas to control the cellular half-life time of proteins resulted in the discovery of the Pasylation concept and foundation of the company XL-protein GmbH. The idea to turn fundamental research results into application allowed CIPSM to establish a strategic alliance with Bayer AG. Prof. Plischke, a former board member at Bayer AG and now member of the supervisory board, is teaching courses on innovation management at CIPSM attended by about 100 graduate students from TUM and LMU. CIPSM was able to submit four successful proposals for new research buildings (Bavarian NMR Center, BioSysM, CPA, and ICEM). This expands the laboratory space on the Campus Großhadern-Martinsried and Garching significantly, with an investment that exceeds 160 million Euros.

Link to the final report

https://dx.doi.org/10.2314/GBV:1696292794

Publications

• CPD Damage Recognition by Transcribing RNA Polymerase II. Science 2007
  Florian Brueckner, Ulrich Hennecke, Thomas Carell, Patrick Cramer
  (See online at https://doi.org/10.1126/science.1135400)
• Functional Architecture of RNA Polymerase I. Cell 2007
  Claus-D. Kuhn, Sebastian R. Geiger, Sonja Baumli, Marco Gartmann, Jochen Gerber, Stefan Jennebach, Thorsten Mielke, Herbert Tschochner, Roland Beckmann, Patrick Cramer
  (See online at https://doi.org/10.1016/j.cell.2007.10.051)
• Mechanism of transcriptional stalling at cisplatindamaged DNA. Nature Structural & Molecular Biology 2007
  Gerke E. Damsma Aaron Alt, Florian Brueckner, Thomas Carell, Patrick Cramer
  (See online at https://doi.org/10.1038/nsmb1314)
• Molecular basis of RNA-dependent RNA polymerase II activity. Nature 2007
  Elisabeth Lehmann, Florian Brueckner, Patrick Cramer
  (See online at https://doi.org/10.1038/nature06290)
• Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7. Science 2007
  Chapman, R.D., Heidemann, M., Albert, T., Mailhammer, R., Flatley, A., Meisterernst, M., Kremmer, E., Eick, D.
  (See online at https://doi.org/10.1126/science.1145977)
• A nano-positioning system for macromolecular structural analysis. Nature Methods 2008
  Adam Muschielok, Joanna Andrecka, Anass Jawhari, Florian Brückner, Patrick Cramer., Jens Michaelis
  (See online at https://doi.org/10.1038/nmeth.1259)
• Clusters of Hyperactive Neurons Near Amyloid Plaques in a Mouse Model of Alzheimer’s Disease. Science 2008
  Busche MA, Eichhoff G, Adelsberger H, Abramowski D, Wiederhold KH, C, Staufenbiel M, Konnerth A, Garaschuk O
  (See online at https://doi.org/10.1126/science.1162844)
• Combined use of RNAi and quantitative proteomics to study gene function in Drosophila. Molecular Cell 2008
  Bonaldi T, Straub T, Kumar C, Cox J, Becker PB, Mann M
  (See online at https://doi.org/10.1016/j.molcel.2008.07.018)
• Mechanoenzymatics of titin kinase. PNAS 2008
  Elias M. Puchner, Alexander Alexandrovich, Ay Lin Kho, Ulf Hensen, Lars V. Schäfer, Birgit Brandmeier, Frauke Gräter, Helmut Grubmüller, Hermann E. Gaub, and Mathias Gautel
  (See online at https://doi.org/10.1073/pnas.0805034105)
• Monitoring Protein Conformation along the Pathway of Chaperonin-Assisted Folding. Cell 2008
  Shruti Sharma, Kausik Chakraborty, Barbara K. Muller, Nagore Astola, Yun-Chi Tang, Don C. Lamb, Manajit Hayer-Hartl, F. Ulrich Hartl
  (See online at https://doi.org/10.1016/j.cell.2008.01.048)
• Single-molecule tracking of mRNA exiting from RNA polymerase II. PNAS 2008
  Joanna Andrecka, Robert Lewis, Florian Brückner, Elisabeth Lehmann, Patrick Cramer, Jens Michaelis
  (See online at https://doi.org/10.1073/pnas.0703815105)
• Structural basis of transcription inhibition by alpha-amanitin and implications for RNA polymerase II translocation. Nature Structural & Molecular Biology 2008
  Brueckner F, Cramer P
  (See online at https://doi.org/10.1038/nsmb.1458)
• Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science 2008
  Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke, B, Cardoso MC, Agard DA, Gustafsson MG, Leonhardt H, Sedat JW
  (See online at https://doi.org/10.1126/science.1156947)
• An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies. PNAS 2009
  D. Schönfeld, G. Matschiner, L. Chatwell, S. Trentmann, H. Gille, M. Hülsmeyer, N. Brown, P. M. Kaye, S. Schlehuber, A. M. Hohlbaum, A. Skerra
  (See online at https://doi.org/10.1073/pnas.0813399106)
• An unfolded CH1 domain controls the assembly and secretion of IgG antibodies. Mol. Cell 2009
  Feige, M.J., Groscurth, S., Marcinowski, M., Shimizu, Y., Kessler, H., Hendershot, L.M. & Buchner, J.
  (See online at https://doi.org/10.1016/j.molcel.2009.04.028)
• Dissection of the ATP- induced conformational cycle of the molecular chaperone Hsp90. Nature Structural & Molecular Biology 2009
  Hessling, M., Richter, K. & Buchner, J.
  (See online at https://doi.org/10.1038/nsmb.1565)
• Ligand-dependent equilibrium fluctuations of single calmodulin molecules. Science 2009
  Junker, J.P., Ziegler, F., Rief, M.
  (See online at https://doi.org/10.1126/science.1166191)
• Thymine Dimerization in DNA Model Systems: Cyclobutane Photolesion Is Predominantly Formed via the Singlet Channel. J. Am. Chem. Soc. 2009
  Julia Kubon, Nadja Regner, Karin Haiser, Tobias E. Schrader, Wolfgang Zinth, Pascale Clivio, Peter Gilch
  (See online at https://doi.org/10.1021/ja900436t)
• ALS‐associated fused in sarcoma (FUS) mutations disrupt Transportin‐ mediated nuclear import. EMBO J. 2010
  Dorothee Dormann, Ramona Rodde, Dieter Edbauer, Eva Bentmann, Ingeborg Fischer, Alexander Hruscha, Manuel E Than, Ian R A Mackenzie, Anja Capell, Bettina Schmid, Manuela Neumann, Christian Haass
  (See online at https://doi.org/10.1038/emboj.2010.143)
• Cilengitide: The First Anti- Angiogenic Small Molecule Drug Candidate. Design, Synthesis and Clinical Evaluation. Anti- Cancer Agents In Medicinal Chemistry 2010
  C. Mas-Moruno, F. Rechenmacher, H. Kessler
  (See online at https://doi.org/10.2174/187152010794728639)
• Dendritic organization of sensory input to cortical neurons in vivo. Nature 2010
  Jia H, Rochefort N, Chen X, Konnerth A
  (See online at https://doi.org/10.1038/nature08947)
• Molecular Basis of RNA Polymerase III Transcription Repression by Maf1. Cell 2010
  Alessandro Vannini, Rieke Ringel, Anselm G. Kusser, Otto Berninghausen, George A. Kassavetis, Patrick Cramer
  (See online at https://doi.org/10.1016/j.cell.2010.09.002)
• Uniform transitions of the general RNA polymerase II transcription complex. Nature Structural & Molecular Biology 2010
  Andreas Mayer, Michael Lidschreiber, Matthias Siebert, Kristin Leike, Johannes Söding, Patrick Cramer
  (See online at https://doi.org/10.1038/nsmb.1903)
• A reversible form of axon damage in experimental autoimmune encephalomyelitis and multiple sclerosis. Nature Medicine 2011
  Nikić I., Merkler D., Sorbara C., Brinkoetter M., Kreutzfeldt M., Bareyre F.M., Brück W., Bishop D., Misgeld T., Kerschensteiner M.
  (See online at https://doi.org/10.1038/nm.2324)
• An efficient synthesis of loline alkaloids. Nature Chemistry 2011
  Mesut Cakmak, Peter Mayer, Dirk Trauner
  (See online at https://doi.org/10.1038/nchem.1072)
• Cytosine methylation alters DNA mechanical properties. Nucleic acids research 2011
  PMD Severin, X Zou, HE Gaub, K Schulten
  (See online at https://doi.org/10.1093/nar/gkr578)
• Functional mapping of single spines in cortical neurons in vivo. Nature 2011
  Chen X, Leischner U, Rochefort NL, Nelken I, Konnerth A
  (See online at https://doi.org/10.1038/nature10193)
• Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF. Nature 2011
  Cameron D. Mackereth, Tobias Madl, Sophie Bonnal, Bernd Simon, Katia Zanier, Alexander Gasch, Vladimir Rybin, Juan Valcárcel, Michael Sattler
  (See online at https://doi.org/10.1038/nature10171)
• Optochemical Genetics. Angewandte Chemie Int. Ed. 2011
  Timm Fehrentz, Matthias Schönberger, Dirk Trauner
  (See online at https://doi.org/10.1002/anie.201103236)
• Proton detected solid-state NMR of fibrillar and membrane proteins. Angew. Chem. Int. Ed. 2011
  Linser R, Dasari M, Hiller M, Higman V, Fink U, Lopez del Amo J-M, Handel L, Kessler B, Schmieder P, Oesterhelt D, Oschkinat H, Reif B
  (See online at https://doi.org/10.1002/anie.201008244)
• RNA polymerase II–TFIIB structure and mechanism of transcription initiation. Nature 2011
  Cheung AC, Cramer P
  (See online at https://doi.org/10.1038/nature09785)
• Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53. Nature structural & molecular biology 2011
  Franz Hagn, Stephan Lagleder, Marco Retzlaff, Julia Rohrberg, Oliver Demmer, Klaus Richter, Johannes Buchner, Horst Kessler
  (See online at https://doi.org/10.1038/nsmb.2114)
• Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins. Nature Structural & Molecular Biology 2011
  Konstantinos Tripsianes, Tobias Madl, Martin Machyna, Dimitrios Fessas, Clemens Englbrecht, Utz Fischer, Karla M Neugebauer, Michael Sattler
  (See online at https://doi.org/10.1038/nsmb.2185)
• Structural basis of RNA polymerase II backtracking, arrest and reactivation. Nature 2011
  Alan C. M. Cheung, Patrick Cramer
  (See online at https://doi.org/10.1038/nature09785)
• Structure and VP16 binding of the Mediator Med25 activator interaction domain. Nature Structural & Molecular Biology 2011
  Erika Vojnic, André Mourão, Martin Seizl, Bernd Simon, Larissa Wenzeck, Laurent Larivière, Sonja Baumli, Karen Baumgart, Michael Meisterernst, Michael Sattler, Patrick Cramer
  (See online at https://doi.org/10.1038/nsmb.1997)
• The C-terminal domain of RNA polymerase II is modified by site-specific methylation. Science 2011
  Sims III, R.J., Rojas, L.A., Beck, D., Bonasio, R., Schüller, R., Drury III, W.J., Eick, D, Reinberg, D.
  (See online at https://doi.org/10.1126/science.1202663)
• The complex folding network of single calmodulin molecules. Science 2011
  Stigler, J., Ziegler, F., Gieseke, A., Gebhardt, J.C., Rief, M.
  (See online at https://doi.org/10.1126/science.1207598)
• A Movie of RNA Polymerase II Transcription. Cell 2012
  Alan C.M. Cheun, Patrick Cramer
  (See online at https://doi.org/10.1016/j.cell.2012.06.006)
• A Photochromic Agonist of AMPA Receptors. Angewandte Chemie Int. Ed. 2012
  Philipp Stawski, Martin Sumser, Dirk Trauner
  (See online at https://doi.org/10.1002/anie.201109265)
• Azo-Propofols: Photochromic Potentiators of GABAA Receptors. Angewandte Chemie Int. Ed. 2012
  Marco Stein, Simon J. Middendorp, Valentina Carta, Ervin Pejo, Douglas E. Raines, Stuart A. Forman, Erwin Sigel, Dirk Trauner
  (See online at https://doi.org/10.1002/anie.201205475)
• CTD Tyrosine Phosphorylation Impairs Termination Factor Recruitment to RNA Polymerase II. Science 2012
  Andreas Mayer, Martin Heidemann, Michael Lidschreiber, Amelie Schreieck, Mai Sun, Corinna Hintermair, Elisabeth Kremmer, Dirk Eick, Patrick Cramer
  (See online at https://doi.org/10.1126/science.1219651)
• CTD tyrosine phosphorylation impairs termination factor recruitment to transcribing RNA polymerase II. Science 2012
  Mayer, A., Heidemann, M., Lidschreiber, M., Schreieck, A., Sun, M., Hintermair, C., Kremmer, E., Eick, D., Cramer, P.
  (See online at https://doi.org/10.1126/science.1219651)
• Immuno- and Constitutive Proteasome Crystal Structures Reveal Differences in Substrate and Inhibitor Specificity. Cell 2012
  Huber E. M., Basler M., Schwab R., Heinemeyer W., Kirk C., Groettrup M., Groll M.
  (See online at https://doi.org/10.1016/j.cell.2011.12.030)
• Label-Free Microscale Thermophoresis Discriminates Sites and Affinity of Protein–Ligand Binding. Angewandte Chemie Int. Ed. 2012
  Susanne A. I. Seidel, Christoph J. Wienken, Sandra Geissler, Moran Jerabek- Willemsen, Stefan Duhr, Alwin Reiter, Dirk Trauner , Dieter Braun, Philipp Baaske
  (See online at https://doi.org/10.1002/anie.201204268)
• Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors. Nature Chemistry 2012
  Ivan Tochitsky, Matthew R. Banghart, Alexandre Mourot, Jennifer Z. Yao, Benjamin Gaub, Richard H. Kramer, Dirk Trauner
  (See online at https://doi.org/10.1038/NCHEM.1234)
• Rapid optical control of nociception with an ionchannel photoswitch. Nature Methods 2012
  Alexandre Mourot, Timm Fehrentz, Yves Le Feuvre, Caleb M Smith, Christian Herold, Deniz Dalkara, Frédéric Nagy, Dirk Trauner, Richard H Kramer
  (See online at https://doi.org/10.1038/NMETH.1897)
• Single-molecule dissection of the high-affinity cohesin– dockerin complex. PNAS 2012
  SW Stahl, MA Nash, DB Fried, M Slutzki, Y Barak, EA Bayer, HE Gaub
  (See online at https://doi.org/10.1073/pnas.1211929109)
• Structural basis of highly conserved ribosome recycling in eukaryotes and archaea. Nature 2012
  Thomas Becker, Sibylle Franckenberg, Stephan Wickles, Christopher J. Shoemaker, Andreas M. Anger, Jean-Paul Armache, Heidemarie Sieber, Charlotte Ungewickell, Otto Berninghausen, Ingo Daberkow, Annette Karcher, Michael Thomm, Karl-Peter Hopfner, Rachel Green, Roland Beckmann
  (See online at https://doi.org/10.1038/nature10829)
• A Conserved RhoGAP Limits M Phase Contractility and Coordinates with Microtubule Asters to Confine RhoA during Cytokinesis. Dev. Cell 2013
  Esther Zanin, Arshad Desai, Ina Poser, Yusuke Toyoda, Cordula Andree, Claudia Moebius, Marc Bickle, Barbara Conradt, Alisa Piekny, Karen Oegema
  (See online at https://doi.org/10.1016/j.devcel.2013.08.005)
• A Pair of Centromeric Proteins Mediates Reproductive Isolation in Drosophila. Species Dev Cell 2013
  Thomae, A.W., Schade, G.O.M., Padeken, J., Borath, M.Vetter,I., Kremmer,E., Heun,P. Imhof,A.
  (See online at https://doi.org/10.1016/j.devcel.2013.10.001)
• ATP-dependent roX RNA remodeling by the helicase maleless enables specific association of MSL proteins. Molecular Cell 2013
  Maenner S, Müller, M, Fröhlich J, Langer D, Becker PB
  (See online at https://doi.org/10.1016/j.molcel.2013.06.011)
• Dynamic readers for 5- (hydroxy)methylcytosine and its oxidized derivatives. Cell 2013
  C.G. Spruijt, F. Gnerlich, A.H. Smits, T. Pfaffeneder, P.W.T.C. Jansen, C. Bauer, M. Münzel, M. Wagner, M. Müller, F. Khan, H.C. Eberl, A. Mensinga, A.B. Brinkman, K. Lephikov, U. Müller, J. Walter, R. Boelens, H. van Ingen, H. Leonhardt, T. Carell
  (See online at https://doi.org/10.1016/j.cell.2013.02.004)
• LBR and Lamin A/C Sequentially Tether Peripheral Heterochromatin and Inversely Regulate Differentiation. Cell 2013
  Solovei I, Wang AS, Thanisch K, Schmidt CS, Krebs S, Zwerger M, Cohen TV, Devys D, Foisner R, Peichl L, Herrmann H, Blum H, Engelkamp D, Stewart CL, Leonhardt H, Joffe B
  (See online at https://doi.org/10.1016/j.cell.2013.01.009)
• NMR Spectroscopy of Soluble Protein Complexes at One Mega‐Dalton and Beyond. Angew. Chem. Int. Ed. 2013
  Mainz, A.; Religa, T.; Sprangers, R.; Linser, R.; Kay, L. E.; Reif, B.
  (See online at https://doi.org/10.1002/anie.201301215)
• Optical control of metabotropic glutamate receptors. Nature Neuroscience 2013
  Joshua Levitz, Carlos Pantoja, Benjamin Gaub, Harald Janovjak, Andreas Reiner, Adam Hoagland, David Schoppik, Brian Kane, Philipp Stawski, Alexander F Schier, Dirk Trauner, Ehud Y Isacoff
  (See online at https://doi.org/10.1038/nn.3346)
• RNA polymerase I structure and transcription regulation. Nature 2013
  Christoph Engel, Sarah Sainsbury, Alan C. Cheung, Dirk Kostrewa, Patrick Cramer
  (See online at https://doi.org/10.1038/nature12712)
• Structural basis of histone H2A-H2B recognition by the essential chaperone FACT. Nature 2013
  Hondele M, Stuwe T, Hassler M, Halbach F, Bowman A, Zhang E, Nijmeijer B, Kotthoff C, Rybin V, Amlacher S, Hurt E, Ladurner AG
  (See online at https://doi.org/10.1038/nature12242)
• Structural mechanism of cytosolic DNA sensing by cGAS. Nature 2013
  Civril F, Deimling T, de Oliveira Mann CC, Ablasser A, Moldt M, Witte G, Hornung V, Hopfner KP
  (See online at https://doi.org/10.1038/nature12305)
• Structures of the human and Drosophila 80S ribosome. Nature 2013
  Andreas M. Anger, Jean-Paul Armache, Otto Berninghausen, Michael Habeck, Marion Subklewe, Daniel N. Wilson, Roland Beckmann
  (See online at https://doi.org/10.1038/nature12104)
• The Backbone Dynamics of the Amyloid Precursor Protein Transmembrane Helix Provides a Rationale for the Sequential Cleavage Mechanism of γ-Secretase. J. Am. Chem. Soc. 2013
  Pester, O., Barrett, P.J., Hornburg, D., Hornburg, P., Proebstle, R., Widmaier, S., Kutzner, C., Duerrbaum, M., Kapurniotu, A., Sanders, C.R., Scharnagl, C., Langosch, D.
  (See online at https://doi.org/10.1021/ja3112093)
• Transcriptome Surveillance by Selective Termination of Noncoding RNA Synthesis. Cell 2013
  Daniel Schulz, Bjoern Schwalb, Anja Kiesel, Carlo Baejen, Phillipp Torkler, Julien Gagneur, Johannes Soeding, Patrick Cramer
  (See online at https://doi.org/10.1016/j.cell.2013.10.024)
• Translation Elongation Factor EF-P Alleviates Ribosome Stalling at Polyproline Stretches. Science 2013
  Susanne Ude, Jürgen Lassak, Agata L. Starosta, Tobias Kraxenberger, Daniel N. Wilson, Kirsten Jung
  (See online at https://doi.org/10.1126/science.1228985)
• Uptake Kinetics and Nanotoxicity of Silica Nanoparticles are Cell Type Dependent. Small 2013
  Blechinger J., Bauer A.T., Torrano A.A., Gorzelanny C., Bräuchle C., Schneider S.W.
  (See online at https://doi.org/10.1002/smll.201301004)
• GroEL/ES Chaperonin Modulates the Mechanism and Accelerates the Rate of TIM-Barrel Domain Folding. Cell 2014
  Georgescauld F, Popova K, Gupta JA, Bracher A, Engen JR, Hayer-Hartl M, Hartl FU
  (See online at https://doi.org/10.1016/j.cell.2014.03.038)
• High susceptibility to fatty liver disease in two-pore channel 2-deficient mice. Nat. Commun. 2014
  Grimm C, Holdt LM, Chen CC, Hassan, H, Jörs S Cuny H, Kissing S, Schröder B, Butz E, Northoff B, Müller C, Spahn S, Castonguay J, Luber CA, Moser M, Lüllmann-Rauch R, Fendel C, Klugbauer N, Griesbeck O, Haas A, Mann M, Bracher F, Teupser D, Saftig P, Biel M, Wahl-Schott C
  (See online at https://doi.org/10.1038/ncomms5699)
• Mass-spectrometry-based draft of the human proteome. Nature 2014
  Wilhelm M, Schlegl J, Hahne H, Gholami AM, Lieberenz M, Savitski MM, Ziegler E, Butzmann L, Gessulat S, Marx H, Mathieson T, Lemeer S, Schnatbaum K, Reimer U, Wenschuh H, Mollenhauer M, Slotta-Huspenina J, Boese JH, Bantscheff M, Gerstmair A, Faerber F, Kuster B
  (See online at https://doi.org/10.1038/nature13319)
• Multi-parametric optical analysis of mitochondrial redox signals during neuronal physiology and pathology in vivo. Nature Medicine 2014
  Breckwoldt M.O., Pfister F., Bradley P.M., Marinkovic P., Williams P.W., Brill M.S., Plomer B., Schmalz A., St Clair D.K., Naumann R., Griesbeck O., Schwarzländer M., Godinho L., Bareyre F.M., Dick T.P., Kerschensteiner M. , Misgeld T.
  (See online at https://doi.org/10.1038/nm.3520)
• Structural basis for the assembly of the Sxl–Unr translation regulatory complex. Nature 2014
  Janosch Hennig, Cristina Militti, Grzegorz M. Popowicz, Iren Wang, Miriam Sonntag, Arie Geerlof, Frank Gabel, Fátima Gebauer, Michael Sattler
  (See online at https://doi.org/10.1038/nature13693)
• Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion. Nature 2014
  Marko Gogala, Thomas Becker, Birgitta Beatrix, Jean- Paul Armache, Clara Barrio-Garcia, Otto Berninghausen, Roland Beckmann
  (See online at https://doi.org/10.1038/nature12950)
• Tet oxidizes thymine to 5-hydroxymethyluracil in mouse embryonic stem cell DNA. Nat. Chem Biol 2014
  Spada, M. Wagner, C. Brandmayr, S. K. Laube, D. Eisen, M. Truss, J. Steinbacher, B. Hackner, O. Kotl-jarova, D. Schuermann, S. Michalakis, O. Kosmatchev, S. Schiesser, B. Steigenberger, N. Raddaoui, G. Kashiwazaki, U. Müller, C. G. Spruijt, M. Vermeulen, H. Leonhardt, P. Schar, M. Müller, T. Carell
  (See online at https://doi.org/10.1038/NCHEMBIO.1532)
• A tunable azine-covalent organic framework platform for light-induced hydrogen generation. Nat. Commun. 2015
  V. S. Vyas, F. Haase, L. Stegbauer, G. Savasci, F. Podjaski, C. Ochsenfeld, B.V.
  (See online at https://doi.org/10.1038/ncomms9508)
• AAA+ chaperones and acyldepsipeptides activate the ClpP protease via conformational control. Nat. Commun. 2015
  Malte Gersch, Kirsten Famulla, Maria Dahmen, Christoph Göbl, Imran Malik, Klaus Richter, Vadim S. Korotkov, Peter Sass, Helga Rübsamen-Schaeff, Tobias Madl, Heike Brötz-Oesterhelt & Stephan A. Sieber
  (See online at https://doi.org/10.1038/ncomms7320)
• An eight-step synthesis of epicolactone reveals its biosynthetic origin. Nature Chemistry 2015
  Pascal Ellerbrock, Nicolas Armanino, Marina K. Ilg, Robert Webster, Dirk Trauner
  (See online at https://doi.org/10.1038/NCHEM.2336)
• Arginine-rhamnosylation as new strategy to activate translation elongation factor P. Nature chemical biology 2015
  Jürgen Lassak, Eva C Keilhauer, Maximilian Fürst, Kristin Wuichet, Julia Gödeke, Agata L Starosta, Jhong-Min Chen, Lotte Søgaard-Andersen, Jürgen Rohr, Daniel N Wilson, Susanne Häussler, Matthias Mann, Kirsten Jung
  (See online at https://doi.org/10.1038/NCHEMBIO.1751)
• DNA repair. Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links. Science 2015
  Räschle M, Smeenk G, Hansen RK, Temu T, Oka Y, Hein MY, Nagaraj N, Long DT, Walter JC, Hofmann K, Storchova Z, Cox J, Bekker-Jensen S, Mailand N, Mann M
  (See online at https://doi.org/10.1126/science.1253671)
• Dynamic DNA devices and assemblies formed by shape-complementary, non-base pairing 3D components. Science 2015
  Gerling, K. Wagenbauer, A. Neuner, H. Dietz
  (See online at https://doi.org/10.1126/science.aaa5372)
• Engulfment path-ways promote programmed cell death by enhancing the unequal segregation of apoptotic potential. Nat. Commun. 2015
  Chakraborty, S., Lambie, E.J., Bindu, S., Mikeladze-Dvali, T., Conradt, B.
  (See online at https://doi.org/10.1038/ncomms10126)
• FAX1, a Novel Membrane Protein Mediating Plastid Fatty Acid Export. PLOS Biology 2015
  Li, N., Gügel, I.L., Giavalsico, P., Zeisler, V., Schreiber, L., Soll, J., Philippar, K.
  (See online at https://doi.org/10.1371/journal.pbio.1002053)
• Fully Blind Peptide-Protein Docking with pepATTRACT. Structure 2015
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