Central facility for mass spectrometry and proteomics

• Applicant: Dr. Andreas Schlosser
• Subject Area: Hematology, Oncology
• Term: from 2013 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 100308792

The central project Z4N will support all projects of the CRU 216 with state-of-the-art mass spectrometry. We will apply quantitative mass spectrometry for the identification of specific protein interaction partners (for example, of Ral proteins), for the identification of cellular targets of small molecule drugs (for example, of dioncoquinones), and for the analysis of newly synthesized proteins in the context of translational regulation. We will set up an advanced workflow for phosphoproteomics and for the selective enrichment of kinases applying a bead-coupled broadly selective kinase inhibitor. We will apply phosphoproteome analyses, for example, for the identification of direct and indirect targets of the kinases NIK and IKK1, and for elucidating MEK-independent signal transduction of Raf kinases.

• DFG Programme: Clinical Research Units
• Subproject of: KFO 216: Characterisation of the Oncogenic Signalling Network in Multiple Myeloma: Development of Targeted Therapies